qPCR EvaGreen® Mastermix
for real-time qPCR and HRM curve analysis…
qPCR EvaGreen® Mastermix (5x, ROX / no ROX) is an optimised ready-to-use 5x solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HotStart Taq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye according to system requirements. The user simply needs to add water, template and primers.
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Volume / Reactions
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Company A
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Company Q
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NatuTec
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5 ml (5x conc.)
► 500x 50 μl reactions
► 2,500x 10 μl reactions
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772.50 €‡
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1,132.50 €‡
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500.00 €
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10 ml (5x conc.)
► 1,000x 50 μl reactions
► 5,000x 10 μl reactions
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1,494.00 €‡
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1,806.00 €‡
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1,000.00 €
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20 ml (5x conc.)
► 2,000x 50 μl reactions
► 10,000x 10 μl reactions
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2,884.00 €‡
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3,612.00 €‡
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2,000.00 €
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‡ Based on price lists 2009.
Comparison among EvaGreen® mastermix and SYBR® Green mastermixes of two leading companies (company A and company Q). Reactions were carried out using an ABI 7900 cycler. The insert is an enlarged view of the area near the baseline for better viewing the curve patterns of the much weaker signals of the two SYBR®-based mastermix.
