Product Review

Special Offer

Bestseller

  

qPCR EvaGreen® Mastermix

for real-time qPCR and HRM curve analysis…

qPCR EvaGreen® Mastermix (5x, ROX / no ROX) is an optimised ready-to-use 5x solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HotStart Taq DNA polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye according to system requirements. The user simply needs to add water, template and primers.

Volume / Reactions

Company A

Company Q

NatuTec

5 ml (5x conc.)
► 500x 50 μl reactions
► 2,500x 10 μl reactions

772.50 €‡

1,132.50 €‡

500.00 €

10 ml (5x conc.)
► 1,000x 50 μl reactions
► 5,000x 10 μl reactions

1,494.00 €‡

1,806.00 €‡

1,000.00 €

20 ml (5x conc.)
► 2,000x 50 μl reactions
► 10,000x 10 μl reactions

2,884.00 €‡

3,612.00 €‡

2,000.00 €

‡ Based on price lists 2009.


Superior sensivity and wide dynamic range

  • EvaGreen® is more sensitive and gives a wider linear detection range than SYBR® Green I.

Low PCR inhibition

  • Exhibits much less PCR inhibition than SYBR® Green I via a smart "release-on-demand" DNA-binding technology.

Compatible with Fast PCR protocol

  • Minimal interference to PCR makes it possible to significantly shorten the chain extension time.

Spectrally similar to SYBR® Green I

  • Compatible with all major brand qPCR instruments.

TaqMan® assay compatible

  • Suitable for TaqMan® assay as well, enabling multidetections by both EvaGreen® dye and oligo probes,followed by melt curve analysis.

Extremely stable

  • Simply indestructible under most biochemical conditions. Can be stored at room temperature and be subject to repeated freeze-thaw cycles.

Compatible with multiplex PCR

  • No dye migration from amplicon to amplicon when used at the recommended concentration.

Nonmutagenic and noncytotoxic


Excellent for HRM application

  • The dye selectively binds to dsDNA via a novel “release on- demand” mechanism that ensures low PCR inhibition and permits high-resolution melt curve analysis (HRM) application at below saturation dye concentration.
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Comparison among EvaGreen® mastermix and SYBR® Green mastermixes of two leading companies (company A and company Q). Reactions were carried out using an ABI 7900 cycler. The insert is an enlarged view of the area near the baseline for better viewing the curve patterns of the much weaker signals of the two SYBR®-based mastermix.

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Tel: 069 – 67 73 78 0 info[at]natutec.de
Fax: 069 – 67 73 78 79 www.natutec.de
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Contact

NatuTec GmbH


Postal Herriotstrasse 1
D-60528 Frankfurt
   
Phone 0180 100 32 72 600-1
Fax 0180 100 32 72 600-2
eMail germany@ebioscience.com