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Catalogue 2007 / 2008
from
Tel: 069 - 67 73 78 0
Fax.: 069 - 67 73 78 79
Email: info@natutec.com |
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 distributed by  has added many new antibodies to its growing range. These new antibodies provide novel useful tools for
- Cancer
- stem cell
- DC and Treg research
Please do not hesitate to call or mail if you have any questions. |
| New Anti-Mouse /Rat Antibodies |
New Anti-Human Antibodies |
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New Anti Mouse Antibodies |
Anti-mouse MIG
The monoclonal antibody MIG-2F5.5 reacts with mouse monokine induced by IFN-g (mig, mig-1, CXCL9). This 14.4 kDa inflammatory chemokine is specifically induced by IFN-g, but not other activators such as LPS or IFN-a. Secretion of mig, mainly by macrophages results in the chemotaxis of a variety of activated T cells via the CXCR3 chemokine receptor. Mig is involved many areas of research including autoimmune diseases, cancer, and inflammation.
Staining of LPS-stimulated (open histogram) and IFN-g-stimulated (colored histogram)
RAW 246.7 cells with PE anti-mouse MIG (MIG-2F5.5).
Total viable cells were used for analysis.
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Anti-mouse/rat 3G11 sialoganglioside antigen
The 3G11 antibody was isolated as an autoantibody against the 3G11 sialylated carbohydrate antigen, which was found to be expressed by thymoyctes and peripheral T cells, but not by B cells, myeloid cells or erythrocytes. 3G11 is expressed at higher levels on CD4+ T cells than on CD8+ T cells, however expression on both of these subsets decreases with advancing age. In thymus, 3G11 is expressed on approximately 20% of total thymocytes. 3G11 has also been used as a marker to distinguish naïve and memory T cells, as 3G11 is irreversibly down-regulated upon activation of CD4+ T cells. The 3G11 antibody is able to block binding of antibodies to CD3ε, suggesting close proximity of these two proteins.
Recently, it has been demonstrated that loss of 3G11 expression in experimental autoimmune encephalomyelitis (EAE) is associated with an anergic, regulatory T phenotype. Purified 3G11- T cells were able to suppress proliferation and cytokine production of 3G11+ T cells, and part of this effect was due to the secretion of IL-10.
 
Staining of C57Bl/6 splenocytes with FITC anti-mouse CD4 (GK1.5) (cat. 11-0041)
and staining buffer (autofluorescence) (left) or 0.25 µg of
Alexa Fluor® 647 anti-mouse 3G11 (SM3G11) (right).
Total viable cells were used for analysis.
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Anti-mouse Endomucin
Endomucin is a 75 kDa type I integral membrane protein, with similarities to the sialomucin family of proteins including extensive O-linked glycosylation. Endomucin is expressed on endothelial cells, however an exception are the high endothelial venules (HEV) of secondary lymphoid organs. In addition, it has been demonstrated that endomucin is expressed on CD34-c-Kit+Sca-1+Lin- hematopoietic progenitors, and that these cells are capable of multi-lineage long-term reconstitution of the hematopoietic compartment.
Staining of bEnd.3 cells with 0.5 µg of Alexa Fluor® 647 Rat IgG2a Iso Cntrl
(cat. 51-4321) (open histogram) or 0.5 µg of Alexa Fluor® 647 anti-mouse Endomucin (V.7C7)
(colored histogram). Total viable cells were used for analysis.
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Anti-mouse SIGN-R1 (C-type lectin, CD209b)
SIGNR1 is a type II transmembrane C-type lectin that was identified in a search for mouse homologues of human DC-SIGN. It is expressed at high levels in splenic marginal zone macrophages and lymph node medullary macrophages, where it functions to uptake dextran polysaccharides, including the capsular polysaccharide of Streptococcus pneumoniae. It has also been demonstrated that SIGNR1 physically associates with TLR4/MD2, and it has been suggested that this association plays a role in recognition of LPS. Furthermore, recently it has been shown that SIGNR1 deficient mice have a defect in catabolism of the complement component C3, and that SIGNR1 binds directly to the complement C1 subcomponent, C1q to assemble a non-conventional C3 convertase.
 
Staining of C57Bl/6 splenocytes with PE anti-mouse CD11b (M1/70) (cat. 12-0112)
and 0.5 µg of Alexa Fluor® 647 Armenian Hamster IgG Iso Cntrl (cat. 51-4888) (left)
or 0.5 µg of Alexa Fluor® 647 anti-mouse SIGN-R1 (eBio22D1) (right).
Cells in the lymphocyte and monocyte gates were used for analysis.
14-2093 |
Affinity Purified anti-mouse SIGN-R1 (C-type lectin, CD209b) |
ELISA,FA,FC,IHC(P),IP |
16-2093 |
Functional Grade* Purified anti-mouse SIGN-R1 (C-type lectin, CD209b) |
ELISA,FA,FC,IHC(P),IP |
51-2093 |
Alexa Fluor® 647 anti-mouse SIGN-R1 (C-type lectin, CD209b) |
FC |
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Anti-mouse Ly108 (SLAM Family receptor)
Mouse Ly108 is also known as NTB-A (NK cell, T cell, B cell antigen), and is expressed on NK, T cells, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and expression of IFN-gamma, and contributes to the inability of NK cells to kill Epstein Barr virus-infected B cells in X-linked lymphoproliferative disease. CD4+ T cells from Ly108 knock-out mice exhibit diminished ability to produce IL-4 and defective neutrophil functions.
Staining has not revealed any strain differences between C57BL/6 and BALB/c mice. Other strains have not been tested.
 
Staining of BALB/c splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452)
and 0.06 µg of PE Mouse IgG2a, K Iso Cntrl (cat. 12-4724) (left) or 0.06 µg of
PE anti-mouse Ly108 receptor (SLAM Family receptor) (right).
Cells in the lymphocyte gate were used for analysis.
12-1508 |
Phycoerythrin (PE) anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108) |
FC |
13-1508 |
Biotin anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108) |
FC |
14-1508 |
Affinity Purified anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108) |
FC IP WB |
16-1508 |
Functional Grade* Purified anti-mouse Ly108 (SLAM Family receptor, also called SLAMF6, TCOM, SF2000, NTBA, NTB-A, NK-T-B-antigen, Ly-108) |
FA FC IP WB |
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New Anti Human Antibodies |
Anti-human TRA-1-60 (Podocalyxin, glycosylation-specific epitope)
The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. The TRA-1-60 epitope can be destroyed by neuroaminidase digestion, unlike the epitope recognized by the related TRA-1-81 antibody. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified in epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Staining of 2102Ep human embryonal carcinoma cells with 0.5 µg of
PE Mouse IgM Iso Cntrl (cat. 12-4752) (open histogram) or 0.5 µg
of PE anti-human TRA-1-60 (colored histogram). Total viable
cells were used for analysis.
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Anti-human TRA-1-81 (Podocalyxin, glycosylation-specific epitope)
The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-81 antibody is lost upon cell differentiation. The TRA-1-81 epitope is resistant to neuroaminidase digestion, unlike the epitope recognized by the related TRA-1-60 antibody. The TRA-1-81 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified in epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Staining of 2102Ep human embryonal carcinoma cells with 0.5 µg of
PE Mouse IgM Iso Cntrl (cat. 12-4752) (open histogram) or
0.5 µg of PE anti-human TRA-1-60 (colored histogram).
Total viable cells were used for analysis.
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Anti-human CD171 (Neurite Cell Adhesion Molecule L1, L1 antigen)
CD171 is a member of the Ig superfamily containing 6 extracellular Ig domains and five fibronectin type III-like repeats. CD171 has been shown to function as a cell adhesion molecule mediating homotypic and heterotypic cell-cell interactions in neuronal myelination, neurite outgrowth and regeneration. Expression of CD171 has been found on monocytes and mature monocytic-derived and follicular DCs, a minor subset of lymphocytes in addition to that found on neuronal tissue and some tumor cells lines. Expression of CD171 on tumors is thought to contribute to tumor progression. Epitope of eBio5G3 is in amino-terminal Ig-like domain.
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Anti-human CD101 (BB27)
CD101 is a disulfide linked homodimer of unknown function. Expression of CD101 is found on monocytes, granulocytes and dendritic cells (Langerhan-like cells HLA-DR, CD1a, CD1c). In addition expression on T lymphocytes is important for cell activation. CD101+ CD28+ cells are very responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can increase proliferation thereby suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit the T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. Recently it has been demonstrated that mouse CD101 is found on a subpopulation of regulatory T cells (CD4, CD25, Foxp3 positive) that have high suppressor activity. Expression of CD101 on human PBMCs shows staining of about 30% of the Foxp3 positive cells. Studies have not confirmed higher suppressor activity in the human CD101 population.
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Anti-human HVEM (herpes virus entry mediator)
HVEM is found on most cell types, including T cells, B cells, monocytes, neutrophils and dendritic cells. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of HVEM was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response. Recent studies have shown HVEM as a unique ligand for BTLA (B and T lymphocyte attenuator). The conservation of the BTLA-HVEM interaction between mouse and human suggests that this system is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response.
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Anti-human HAI-1 (HGF activator inhibtor-1)
HAI-1 is a single transmembrane protein of approximately 53 kDa with an extracellular Kunitz-type serine protease inhibitor domain. HAI-1 and HAI-2 are produced in membrane-associated forms, which are secreted as active, proteolytically truncated proteins. HAI can bind to and inhibit HGFA and matripase, which are responsible for converting HGF to an active form. Human HAI-1 transcript is expressed in various human tissues, such as adult placenta, kidney, pancreas, prostate, small intestine, fetal kidney and fetal lung. HAI-1 is a marker for prostate and breast cancers.
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Functional Grade (FG) Purified monoclonal antibodies
for functional Assays
Studies in the mouse model using both in vitro and in vivo assays have resulted in better understanding of the immune function and the role of different cell types in the immune response. In vitro functional assays offer the advantage of a minimalist approach, where conditions including type of cell and stimulations can be limited by design. Functions of different cell types such as
T- or B cells, direct interactions between two cell types and intracellular signaling pathways initiated by a specific stimulus can be predominantly studied in vitro.
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Experiments performed in a living laboratory mouse, however, provide information about the direct biological relevance, collaboration, and interdependence of complex biological processes, many of which may not be well understood.
Antibodies are used for in vivo studies where the injected antibody is intended to either deplete a specific cell type or induce activation of cells. NatuTec offers with eBioscience monoclonal antibodies to mouse and human cell surface antigens and cytokines for in vitro or in vivo functional studies.
For functional studies, we recommend using Functional Grade (FG) Purified monoclonal antibodies. FG Purified antibodies are sterile, contain verified low level of endotoxin (<0.001 ng/µg) and do not have sodium azide (NaN3) or any other preservatives.
Download the complete list of FG purified antibodies as pdf by clicking on the picture above or send a mail for your personal hardcopy. |
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FoxP3 characterisation studies of regulative T-cells |
FoxP3 antibodies for Flowcytometry, Western blot, Immunohistochemistry
for human, Mouse, Rat, and non human primates
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| Toll-like receptors |
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Antibodies qualified for use with
Formalin-Fixed Paraffin-Embedded
(FFPE) Tissues.
Human and mouse tissues tested.
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Aktive Kinasen für die Untersuchung der Signaltransduktion
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Tandem und Trippel Konjugate, Anti-human CDs |
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Gereinigte IgGs aus zahlreichen Tierspezies |
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Mouse Cytokin ELISAs
GM-CSF, IFN-g, IFN-g Femto, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12/IL-23, IL-23, MCP-1, TNF-a
Human Cytokin ELISAs
Granzyme B, IFN-g, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 p70m, MCP-1, TNF-a, TNF-b
Rat Cytokin ESLISAs
IFN-g, IL-1b, TNF-a
Ready Set Go ELISA! Protokoll |
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